Quantitative Studies on the Fixation Ratio "Complement: Antibody" in Complement-Fixation Tests with Syphilitic Serum
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چکیده
منابع مشابه
Studies on Complement Fixation
It is shown by complement fixation studies with protein antigens and specific immune rabbit sera that the rate of fixation of complement is determined by the concentration of antibodies in the immune sera, that the greater part of fixation of complement takes place during the 1st hour, and that fixation is practically completed at the end of 4 hours at ice box temperature. It is further shown t...
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Bordet and Gengou (1), in 1906, performed serological tests on pertussis employing the organisms which they discovered and claimed to be the causative agent of whooping cough as the antigen for the complement fixation test. They got a positive reaction in most cases of the disease. Yet, later investigations failed to confirm these results. Among those who confirmed their results were Renaux (2)...
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Infection with poliomyelitis virus gives rise to complement-fixing (C-F) antibodies which may be detected with antigens prepared either from the brains of infected mice or tissue culture fluids, as shown with Type II by Casals et al."' and with all three types by Svedmyr et al.' These workers used sufficient antigen in each test to obtain maximum sensitivity. With this antigen concentration the...
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A quantitative micro-complement fixation test capable of detecting nanogram quantities of antigen is described. The test is simple to perform and is highly reproducible. Typical results from three antigen-antibody systems are given.
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The prolonged extraction of the tubercle bacillus with boiling ethyl alcohol, followed by one or more reprecipitations by chilling the hot alcoholic solution, easily yields a preparation very active as antigen in the complement fixation reaction. This preparation gives a precipitation reaction with high dilution of the normal blood serum of a number of species. The precipitation reaction presen...
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ژورنال
عنوان ژورنال: Sexually Transmitted Infections
سال: 1962
ISSN: 1368-4973
DOI: 10.1136/sti.38.1.33